Proteintech, 80067-1-RR, Phospho-Beta Catenin (Ser33) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Beta Catenin (Ser33) (80067-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Beta Catenin (Ser33) in WB, ELISA, FC (Intra) applications with reactivity to Human, Mouse, Rat samples 80067-1-RR targets Phospho-Beta Catenin (Ser33) in WB, IF, FC (Intra), ELISA applications and shows reactivity with Human, Mouse, Rat samples. Tested Applications Positive WB detected in: PC-3 cells, HT-29 cells, Calyculin A treated HT-29 cells, Calyculin A treated PC-3 cells Positive FC (Intra) detected in: Calyculin A treated PC-3 cells, PC-3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information β-Catenin, also known as CTNNB1, is an evolutionarily conserved, multifunctional intracellular protein. β-Catenin was originally identified in cell adherens junctions (AJs) where it functions to bridge the cytoplasmic domain of cadherins to a-catenin and the actin cytoskeleton. Besides its essential role in the AJs, β-catenin is also a key downstream component of the canonical Wnt pathway that plays diverse and critical roles in embryonic development and adult tissue homeostasis. The Wnt/β-catenin pathway is also involved in the activation of other intracellular messengers such as calcium fluxes, JNK, and SRC kinases. Deregulation of β-catenin activity is associated with multiple diseases including cancers. (PMID: 22617422; 18334222). CK1 phosphorylates β-Catenin at Ser45. This phosphorylation event primes β-Catenin for subsequent phosphorylation by GSK-3β. GSK-3β destabilizes β-catenin by phosphorylating it at Ser33, Ser37, and Thr41. Mutations at these sites result in the stabilization of β-Catenin protein levels and have been found in many tumor cell lines . Specification Tested Reactivity: Human, Mouse, Rat Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: catenin (cadherin-associated protein), beta 1, 88kDa Calculated Molecular Weight: 781 aa, 86 kDa Observed Molecular Weight: 90 kDa GenBank Accession Number: BC058926 Gene Symbol: Beta Catenin Gene ID (NCBI): 1499 ENSEMBL Gene ID: ENSG00000168036 RRID: AB_2918861 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P35222 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.