Proteintech, 80077-1-RR, P53 Recombinant monoclonal antibody

Size: 20ul / 100ul The P53 (80077-1-RR) by Proteintech is a Recombinant antibody targeting P53 in WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA applications with reactivity to human, rat, zebrafish samples 80077-1-RR targets P53 in WB, IHC-Autostainer, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human, rat, zebrafish samples. Tested Applications Positive WB detected in: COLO 320 cells, A431 cells, HSC-T6 cells, HT-29 cells, ROS1728 cells, SW480 cells, zebrafish tissue Positive IHC-Autostainer detected in: human colon cancer tissue, human ovarian cancer Positive IF/ICC detected in: HepG2 cells, A431 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC)-AUTOSTAINER: IHC-AUTOSTAINER : 1:50-1:500 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information TP53, also known as P53 and NY-CO-13, belongs to the p53 family and has 9 isoforms. In SDS-Page, the observed molecular weight is about 53 kDa. TP53 acts as a tumor suppressor in many tumor types, including growth arrest or apoptosis depending on the physiological circumstances and cell types. It is involved in cell cycle regulation as a trans-activator that acts to negatively regulate cell division by controlling a set of genes required for this process. TP53 Localizes in the nucleus in most cells but found in the cytoplasm in some cells. (PMID: 26166714; PMID: 25225161) Specification Tested Reactivity: human, rat, zebrafish Cited Reactivity: human, mouse, zebrafish Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tumor protein p53 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC003596 Gene Symbol: P53 Gene ID (NCBI): 7157 RRID: AB_2918862 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P04637 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.