Proteintech, 80077-9-RR, Phospho-P53 (Ser46) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-P53 (Ser46) (80077-9-RR) by Proteintech is a Recombinant antibody targeting Phospho-P53 (Ser46) in WB, IF/ICC, ELISA applications with reactivity to human samples 80077-9-RR targets Phospho-P53 (Ser46) in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: etoposide treated HT-29 cells, nocodazole treated A431 cells Positive IF/ICC detected in: etoposide treated HT-29 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information The p53 tumor suppressor protein plays a major role in cellular response to DNA damage and other genomic aberrations. Activation of p53 can lead to either cell cycle arrest and DNA repair or apoptosis. Phosphorylation of p53 at Ser46 regulates the ability of p53 to induce apoptosis. Homeodomain-interacting protein kinase 2 (HIPK2) Phosphorylates Ser46 off p53 and p38 can phosphorylate this site. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tumor protein p53 Calculated Molecular Weight: 44 kDa Observed Molecular Weight: 53 kDa GenBank Accession Number: BC003596 Gene Symbol: P53 Gene ID (NCBI): 7157 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P04637 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.