Proteintech, 80086-1-RR, Phospho-JUN (Ser73) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-JUN (Ser73) (80086-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-JUN (Ser73) in WB, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications with reactivity to human, mouse samples 80086-1-RR targets Phospho-JUN (Ser73) in WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: UV treated NIH/3T3 cells Positive IF/ICC detected in: UV (1 hour) and 100 nM Calyculin A (30 minutes) HeLa cells Positive FC (Intra) detected in: UV treated NIH/3T3 cells Positive ChIP-qPCR detected in: hβ-NGF (starved overnight) (50 ng/ml, 2 h) PC-12 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information JUN, the most extensively studied protein of the activator protein-1 (AP-1) complex, is involved in numerous cell activities, such as proliferation, apoptosis, survival, tumorigenesis and tissue morphogenesis (PMID: 22180088). JUN is a transcription factor that recognizes and binds to the enhancer heptamer motif 5'-TGA[CG]TCA-3'. It promotes activity of NR5A1 when phosphorylated by HIPK3 leading to increased steroidogenic gene expression upon cAMP signaling pathway stimulation. JUN is a basic leucine zipper (bZIP) transcription factor that acts as homo- or heterodimer, binding to DNA and regulating gene transcription (PMID: 9732876). In additon, extracellular signals can induce post-translational modifications of JUN, resulting in altered transcriptional activity and target gene expression (PMID:8464713). More over, it has uncovered multiple layers of a complex regulatory scheme in which JUN is able to crosstalk, amplify and integrate different signals for tissue development and disease. Jun is predominantly nuclear, ubiquitinated Jun colocalizes with lysosomal proteins (PMID: 15469925). This antibody is raised against synthetic phosphopeptide corresponding to residues surrounding Ser73 of human JUN, which can detect the bands around 42-45 kDa. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, chicken Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: jun oncogene Calculated Molecular Weight: 331 aa, 36 kDa Observed Molecular Weight: 42-45 kDa GenBank Accession Number: BC068522 Gene Symbol: JUN Gene ID (NCBI): 3725 RRID: AB_3085353 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P05412 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.