Proteintech, 80093-1-RR, Phospho-MST1 (Thr183)/MST2 (Thr180) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-MST1 (Thr183)/MST2 (Thr180) (80093-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 80093-1-RR targets Phospho-MST1 (Thr183)/MST2 (Thr180) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Jurkat cells, HeLa cells, Calyculin A treated NIH/3T3 cells, Calyculin A treated HeLa cells, Staurosporine treated Ramos cells, Staurosporine treated Jurkat cells Positive FC (Intra) detected in: Calyculin A treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Mammalian STE20-like serine-threonine kinase MST1, encoded by the STK4 gene, is a multifunctional protein. MST1 and its closest paralogs MST2 (encoded by the STK3 gene), MST3, and MST4 are members of the Class II Germinal Center Family of Protein Kinases . STK3/4 and LATS1/2 (large tumor suppressor 1 and 2) are core kinase components of the Hippo tumor suppressor pathway in mammalians . In the conventional Hippo pathway, the STK3/4 and LATS1/2 signaling cascade phosphorylates and inactivates the transcriptional coactivator YAP1 (yes associated protein 1) and its close paralog WWTR1]. YAP1 and WWTR1 do not have DNA binding domains and they exert their biological outputs, such as cell proliferation and survival, by interacting with the TEAD1-4 transcription factors. Lines of evidence have indicated that dysregulation or loss of STK4/Hippo signaling is linked to developmental disorders and carcinogenesis with poor prognosis. STK4 is a stress-induced kinase and it can be activated in response to cell-death inducers. Autophosphorylation of STK4 at Thr183 (Thr180 in STK3) in the activation loop is a key activation mechanism for STK4/3 because phosphorylation of Thr183/180 causes the cleavage of STK4 by caspases under apoptotic conditions. The caspase-cleavage results in a more active STK4 protein (STK4-N, an amino-terminally truncated STK4), which localizes into the nucleus and induces apoptosis through histone modifications and chromatin condensations. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: serine/threonine kinase 4 Calculated Molecular Weight: 56 kDa Observed Molecular Weight: 59 kDa GenBank Accession Number: BC005231 Gene Symbol: MST1 Gene ID (NCBI): 6789 RRID: AB_2918864 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13043 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.