Proteintech, 80209-6-RR, Phospho-AMPK Alpha (Thr172) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-AMPK Alpha (Thr172) (80209-6-RR) by Proteintech is a Recombinant antibody targeting Phospho-AMPK Alpha (Thr172) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 80209-6-RR targets Phospho-AMPK Alpha (Thr172) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: H2O2 treated C2C12 cells, λ phosphatase treated HEK-293 cells Positive FC (Intra) detected in: λ phosphatase treated HEK-293 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information AMPK is a serine/threonine protein kinase complex consisting of a catalytic α-subunit (α1 and α2), a scaffolding β-subunit (β1 and β2), and a regulatory γ-subunit (γ1, γ2, and γ3). Ubiquitous expression of AMPKα1-, β1-, and γ1-subunits in many tissues makes the α1β1γ1 complex a reference for AMPK assays to identify AMPK activators. AMPK is generally quiescent under normal conditions but is activated in response to signals and stresses that increase the AMP/ATP ratio, such as hypoglycemia, strenuous exercise, anoxia, and ischemia. An increase in the ratio of AMP/ATP activates AMPK by several mechanisms, including direct allosteric activation and covalent modification in which an AMP-dependent AMPK kinase (AMPKK) phosphorylates the α subunit on Thr172. Once activated, AMPK switches on catabolic pathways that generate ATP, while switching off ATP-consuming processes (e.g., biosynthesis, cell growth, and proliferation), and in doing so acts as an "energy gauge". (PMID: 27034026, PMID: 21980456, PMID: 27600021)This antibody can recognize the phosphorylation sites of Thr183 in AMPK Alpha 1 and Thr172 in AMPK Alpha 2. Specification Tested Reactivity: human, mouse Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: protein kinase, AMP-activated, alpha 1 catalytic subunit Observed Molecular Weight: 64 kDa GenBank Accession Number: BC048980 Gene Symbol: AMPK Alpha 1 Gene ID (NCBI): 5562 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13131 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.