Proteintech, 80218-1-RR, Phospho-ULK1 (Ser556) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-ULK1 (Ser556) (80218-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-ULK1 (Ser556) in WB, FC (Intra), ELISA applications with reactivity to human samples 80218-1-RR targets Phospho-ULK1 (Ser556) in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: PC-3 cells, HeLa cells, Calyculin A treated HeLa cells, Calyculin A treated PC-3 cells Positive FC (Intra) detected in: Calyculin A treated PC-3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.50 ug per 10^6 cells in a 100 µl suspension Background Information Unc-51-like-kinase 1 (ULK1) is a target of both the mechanistic target of rapamycin (mTOR) and AMP activated protein kinase (AMPK), whose role is to facilitate the initiation of autophagy in response to starvation. ULK1 is phosphorylated on serine 638 and 758 sites by mTOR in nutrient-rich conditions, inhibiting ULK1 activation by disrupting its binding to AMPK. Upon glucose starvation, dissociation of mTOR from ULK1 and phosphorylation by AMPK leads to the activation of ULK1 activity. The S556 site of ULK1 is one of the major AMPK-dependent phosphorylation sites. (PMID: 30517873, PMID: 21258367) Specification Tested Reactivity: human Cited Reactivity: human, mouse, rat, goat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: unc-51-like kinase 1 (C. elegans) Observed Molecular Weight: 140 kDa GenBank Accession Number: NM_003565 Gene Symbol: ULK1 Gene ID (NCBI): 8408 RRID: AB_2918877 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O75385 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.