Proteintech, 80346-1-RR, Phospho-Caspase 9 (Ser196) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Caspase 9 (Ser196) (80346-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-Caspase 9 (Ser196) in WB, IF/ICC, ELISA applications with reactivity to human, mouse samples 80346-1-RR targets Phospho-Caspase 9 (Ser196) in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: NIH/3T3 cells, HEK-293 cells, Jurkat cells, Calyculin A treated HEK-293 cells, Calyculin A treated Jurkat cells, Calyculin A treated NIH/3T3 cells Positive IF/ICC detected in: Calyculin A treated HeLa cells Positive FC (Intra) detected in: Calyculin A treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information Caspase 9 also name as MCH6, APAF3, APAF-3, ICE-LAP6 and CASPASE-9c, is a member of the cysteine-aspartic acid protease (caspase) family. It's synthesized as a 46kDa precursor protein which can be cleaved into a 35kDa subunit and a 11kDa subunit. The phosphorylated type can be detected at 55kDa and 35kDa. It plays a central role in the mitochondrial or intrinsic apoptotic pathway that is engaged in response to many apoptotic stimuli. Once activated, caspase-9 cleaves and activates the effector caspases 3 and 7 to bring about apoptosis. It can be phosphorylated by PKB/AKT1 at Ser196, this modification will downregulate its activity and decrease apoptosis. Akt phosphorylation site found in human caspase 9 is absent in mouse caspase 9.It's reported that there is an increase in caspase 9 expression and activity in the hypoxic brain. Inhibition of Caspase 9 activity would render opportunity to treat neurological diseases such as stroke, neurodegenerative diseases or brain injury caused by hypoxia. (PMID: 19788417, PMID: 10529400, PMID: 9812896, PMID: 18840507) In recent years, the localization of caspase9 was a focus of interest. Beside its cytoplasmic distribution, a very extensive localization study was done on rat brain tissue, where caspase9 was found located predominantly in the nucleus and to a lesser extend in the cytoplasm [PMID: 15541731]. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: caspase 9, apoptosis-related cysteine peptidase Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 46 kDa, 35 kDa GenBank Accession Number: BC002452 Gene Symbol: Caspase 9 Gene ID (NCBI): 842 RRID: AB_2918890 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P55211 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.