Proteintech, 80553-2-RR, Phospho-TSC2 (Ser1387) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-TSC2 (Ser1387) (80553-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-TSC2 (Ser1387) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 80553-2-RR targets Phospho-TSC2 (Ser1387) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Calyculin A treated NIH/3T3 cells, NIH/3T3 cells Positive FC (Intra) detected in: Calyculin A treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information TSC2, also named as Tuberin, plays an essential role in the cellular energy response pathway. TSC2 forms a physical and functional complex with TSC1 that can inhibit the nutrient-mediated or growth factor-stimulated phosphorylation of S6K1 and EIF4EBP1 by negatively regulating mTORC1 signaling. AMPK can phosphorylate and activate the Ser1387 site of TSC2.(PMID: 14651849, 12172553, PMID: 23244622) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: tuberous sclerosis 2 Calculated Molecular Weight: 1807 aa, 201 kDa Observed Molecular Weight: 200 kDa GenBank Accession Number: BC150300 Gene Symbol: TSC2 Gene ID (NCBI): 7249 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P49815 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.