Proteintech, 80735-1-RR, HSP70 Recombinant monoclonal antibody

Size: 20ul / 100ul The HSP70 (80735-1-RR) by Proteintech is a Recombinant antibody targeting HSP70 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, yeast samples 80735-1-RR targets HSP70 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, yeast samples. Tested Applications Positive WB detected in: HEK-293 cells, human urine exosomes, HeLa cells, K-562 cells, NIH/3T3 cells, C6 cells, the whole yeast, HEK-293 exosomes Positive IHC detected in: human breast cancer tissue, human colon cancer tissue, human liver cancer tissue, human lung squamous cell carcinoma tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:20000 Immunohistochemistry (IHC): IHC : 1:200-1:6000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information HSPA1A , collectively known as HSP70 (also referred to HSP72), is a stress-inducible member of heat-shock protein 70 (HSP70) proteins which are highly conserved chaperons implicated in protein folding, protein refolding, protein transport, and protein targeting. Encoded by two closely linked, intronless and stress-inducible genes, HSPA1A and HSPA1B differ by only two amino acids and are believed to be fully interchangeable proteins. HSPA1A is a cytosol nuclear protein able to translocate between cytoplasm and nucleus. Generally, HSPA1A is thought to be expressed in unstressed normal cells at low or undetectable levels. Expression of HSPA1A protein can be highly activated by various stressful stimuli. Significant up-regulation of HSPA1A has been found in various tumors. Recently it has been reported that HSPA1A can be constitutively expressed in selected cell types. HSP70 is also used as exosomal marker Specification Tested Reactivity: human, mouse, rat, yeast Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1446 Product name: Recombinant human HSP70 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 291-641 aa of BC009322 Sequence: IDFYTSITRARFEELCSDLFRSTLEPVEKALRDAKLDKAQIHDLVLVGGSTRIPKVQKLLQDFFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSENVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFELSGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKANKITITNDKGRLSKEEIERMVQEAEKYKAEDEVQRERVSAKNALESYAFNMKSAVEDEGLKGKISEADKKKVLDKCQEVISWLDANTLAEKDEFEHKRKELEQVCNPIISGLYQGAGGPGPGGFGAQGPKGGSGSGPTIEEVD Predict reactive species Full Name: heat shock 70kDa protein 1A Calculated Molecular Weight: 70 kDa Observed Molecular Weight: 70 kDa GenBank Accession Number: BC009322 Gene Symbol: HSP70 Gene ID (NCBI): 3303 RRID: AB_2918908 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P0DMV8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.