Proteintech, 80771-2-RR, Phospho-BCL2 (Ser70) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-BCL2 (Ser70) (80771-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-BCL2 (Ser70) in WB, IHC, FC (Intra), ELISA applications with reactivity to human samples 80771-2-RR targets Phospho-BCL2 (Ser70) in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Jurkat cells, paclitaxel treated Jurkat cells, Calyculin A treated Jurkat cells Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: Staurosporine treated Jurkat cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension Background Information BCL2 belongs to the Bcl-2 family. The anti-apoptotic protein BCL2 can bind to the pro-apoptotic protein BAX or BAK1 to form heterodimers that modulate apoptosis. BCL2 phosphorylation at Ser70 enhances binding to BAX and BAD and is essential for its anti-apoptotic activity. Conversely, BCL2 also regulates autophagy via interaction with BECN1/Beclin 1; moreover, BCL2 phosphorylation including at Ser70 leads to its dissociation from BECN1 and induction of autophagy. These findings suggest that BCL2 regulates the balance between apoptosis and autophagy. (PMID: 29433359) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: B-cell CLL/lymphoma 2 Calculated Molecular Weight: 26 kDa Observed Molecular Weight: 26 kDa GenBank Accession Number: BC027258 Gene Symbol: BCL2 Gene ID (NCBI): 596 RRID: AB_3670487 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P10415 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.