Proteintech, 80801-2-RR, DYKDDDDK tag Recombinant monoclonal antibody (Binds to FLAG® tag epitope)

Size: 100ul The DYKDDDDK tag (80801-2-RR) by Proteintech is a Recombinant antibody targeting DYKDDDDK tag in WB, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to recombinant protein samples 80801-2-RR targets DYKDDDDK tag in WB, IHC, IF/ICC, FC (Intra), IP, CoIP, ELISA applications and shows reactivity with recombinant protein samples. Tested Applications Positive WB detected in: TDP-43-flag Transfected HEK-293T cells Positive IP detected in: Transfected HEK-293 cells Positive IF/ICC detected in: Transfected HEK-293T cells Positive FC (Intra) detected in: Transfected HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Protein tags are protein or peptide sequences located either on the C- or N- terminal of the target protein, which facilitates one or several of the following characteristics: solubility, detection, purification, localization and expression. The DYKDDDDK(FLAG) peptide has been used extensively as a general tag in expression vectors. This peptide can be expressed and detected with the protein of interest as an amino-terminal or carboxy-terminal fusion. N-terminal DDDDK vectors provide an Ek cleavage site for removal of the fusion tag. The DDDDK peptide is likely to be located on the surface of a fusion protein because of its hydrophilic nature. As a result, the DDDDK peptide is more likely to be accessible to antibodies. A DDDDK-tag can be used in many different assays that require recognition by an antibody, such as western blotting, immunocytochemistry, immunoprecipitation, flow cytometry, protein purification, and in the study of protein-protein interactions, cell ultrastructure, and protein localization and so on. This antibody is a rabbit recombinant antibody raised against 3xFlag (3xDYKDDDDKT) sequence and recognizes the (1x) and (3x)DYKDDDDK peptide and detects DDDDK-tagged proteins. Anti-FLAG is a registered trademark of Sigma-Aldrich Biotechnology. Specification Tested Reactivity: recombinant protein Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2329 Product name: Recombinant DYKDDDDK tag protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: flag of Sequence: DYKDDDDK Predict reactive species Full Name: DYKDDDDK tag RRID: AB_3670488 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: FLAGTAG Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.