Proteintech, 80849-1-RR, GRP78/BIP Recombinant monoclonal antibody

Size: 20ul / 100ul The GRP78/BIP (80849-1-RR) by Proteintech is a Recombinant antibody targeting GRP78/BIP in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat, pig samples 80849-1-RR targets GRP78/BIP in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: HepG2 cells Positive IP detected in: HeLa cells Positive IHC detected in: human colon cancer tissue, human pancreas cancer tissue, human breast cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:2000-1:8000 Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: pig Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2188 Product name: Recombinant human GRP78,BIP protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 351-654 aa of BC020235 Sequence: LKKSDIDEIVLVGGSTRIPKIQQLVKEFFNGKEPSRGINPDEAVAYGAAVQAGVLSGDQDTGDLVLLDVCPLTLGIETVGGVMTKLIPRNTVVPTKKSQIFSTASDNQPTVTIKVYEGERPLTKDNHLLGTFDLTGIPPAPRGVPQIEVTFEIDVNGILRVTAEDKGTGNKNKITITNDQNRLTPEEIERMVNDAEKFAEEDKKLKERIDTRNELESYAYSLKNQIGDKEKLGGKLSSEDKETMEKAVEEKIEWLESHQDADIEDFKAKKKELEEIVQPIISKLYGSAGPPPTGEEDTAEKDEL Predict reactive species Full Name: heat shock 70kDa protein 5 (glucose-regulated protein, 78kDa) Calculated Molecular Weight: 78 kDa Observed Molecular Weight: 70,78 kDa GenBank Accession Number: BC020235 Gene Symbol: GRP78/BIP Gene ID (NCBI): 3309 RRID: AB_2918916 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P11021 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.