Proteintech, 80911-4-RR, Fibronectin Recombinant monoclonal antibody

Size: 20ul / 100ul The Fibronectin (80911-4-RR) by Proteintech is a Recombinant antibody targeting Fibronectin in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 80911-4-RR targets Fibronectin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: NIH/3T3 cells, HepG2 cells, rat plasma, mouse uterus tissue Positive IF/ICC detected in: NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Background Information Fibronectin (fibronectin) is a kind of macromolecular glycoprotein widely existing in extracellular matrix (ECM), plasma and various cell surfaces, which plays a central role in key biological processes such as cell adhesion, migration, proliferation, differentiation, tissue repair and embryo development. Fibronectin is usually a dimer structure, which consists of two subunits connected by disulfide bonds. There are two main forms of Fibronectin: plasma Fibronectin(pFN) is synthesized by liver cells and exists in the blood, which is involved in the processes of coagulation and wound repair. Cellular Fibronectin(cFN) is synthesized by fibroblasts and other cells, deposited in extracellular matrix, and participates in the interaction between cells and matrix. Its molecular weight ranges from 220 to 275 kDa. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg5117 Product name: Recombinant Human Fibronectin protein (rFc Tag) Source: mammalian cells -derived, pHZ-KIsec-C-rFc Tag: C-rFc Domain: 607-1265 aa of NM_212482.4 Sequence: SSSGPVEVFITETPSQPNSHPIQWNAPQPSHISKYILRWRPKNSVGRWKEATIPGHLNSYTIKGLKPGVVYEGQLISIQQYGHQEVTRFDFTTTSTSTPVTSNTVTGETTPFSPLVATSESVTEITASSFVVSWVSASDTVSGFRVEYELSEEGDEPQYLDLPSTATSVNIPDLLPGRKYIVNVYQISEDGEQSLILSTSQTTAPDAPPDTTVDQVDDTSIVVRWSRPQAPITGYRIVYSPSVEGSSTELNLPETANSVTLSDLQPGVQYNITIYAVEENQESTPVVIQQETTGTPRSDTVPSPRDLQFVEVTDVKVTIMWTPPESAVTGYRVDVIPVNLPGEHGQRLPISRNTFAEVTGLSPGVTYYFKVFAVSHGRESKPLTAQQTTKLDAPTNLQFVNETDSTVLVRWTPPRAQITGYRLTVGLTRRGQPRQYNVGPSVSKYPLRNLQPASEYTVSLVAIKGNQESPKATGVFTTLQPGSSIPPYNTEVTETTIVITWTPAPRIGFKLGVRPSQGGEAPREVTSDSGSIVVSGLTPGVEYVYTIQVLRDGQERDAPIVNKVVTPLSPPTNLHLEANPDTGVLTVSWERSTTPDITGYRITTTPTNGQQGNSLEEVVHADQSSCTFDNLSPGLEYNVSVYTVKDDKESVPISDTIIP Predict reactive species Full Name: fibronectin 1 Calculated Molecular Weight: 272 kDa Observed Molecular Weight: 220-275 kDa GenBank Accession Number: NM_212482.4 Gene Symbol: Fibronectin Gene ID (NCBI): 2335 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P02751-15 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.