Proteintech, 81004-1-RR, LC3B Recombinant monoclonal antibody

Size: 20ul / 100ul The LC3B (81004-1-RR) by Proteintech is a Recombinant antibody targeting LC3B in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat, pig samples 81004-1-RR targets LC3B in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat, pig samples. Tested Applications Positive WB detected in: mouse brain tissue, HEK-293 cells, HeLa cells, Chloroquine treated HEK-293 cells, Chloroquine treated HeLa cells Positive IHC detected in: human colon cancer tissue, mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: Chloroquine treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information Map1LC3, also known as LC3, is the human homolog of yeast Atg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC3 isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18 kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 14-16 kDa form that is associated with the autophagosome membrane. The antibody 81004-1-RR specifically recognizes LC3B. Specification Tested Reactivity: human, mouse, rat, pig Cited Reactivity: human, mouse, rat, bovine, sheep Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag6144 Product name: Recombinant human LC3 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-125 aa of BC067797 Sequence: MPSEKTFKQRRTFEQRVEDVRLIREQHPTKIPVIIERYKGEKQLPVLDKTKFLVPDHVNMGELIKIIRRRLQLNANQAFFLLVNGHSMVSVSTPISEVYESEKDEDGFLYMVYASQETFGMKLSV Predict reactive species Full Name: microtubule-associated protein 1 light chain 3 beta Calculated Molecular Weight: 15 kDa Observed Molecular Weight: 14-18 kDa GenBank Accession Number: BC067797 Gene Symbol: LC3B Gene ID (NCBI): 81631 ENSEMBL Gene ID: ENSG00000140941 RRID: AB_2923695 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q9GZQ8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.