Proteintech, 81011-1-RR, OXCT1 Recombinant monoclonal antibody

Size: 20ul / 100ul The OXCT1 (81011-1-RR) by Proteintech is a Recombinant antibody targeting OXCT1 in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 81011-1-RR targets OXCT1 in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, mouse skeletal muscle, mouse brain, rat brain, mouse heart, rat heart Positive IF/ICC detected in: HeLa cells, SKOV-3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000 Background Information 3-oxoacid-CoA transferase 1 (OXCT1), encoded by nuclear gene, is a mitochondrial CoA transferase required for ketone body degradation. It catalyzes the transfer of CoA from succinyl-CoA to acetoacetate, generating acetoacetyl-CoA. OXCT1 is expressed in brain, heart, and skeletal muscle, but not in liver. This antibody specifically recognizes endogenous OXCT1. (21209089) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag2818 Product name: Recombinant human SCOT protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 151-520 aa of BC009001 Sequence: GVPAFYTPTGYGTLVQEGGSPIKYNKDGSVAIASKPREVREFNGQHFILEEAITGDFALVKAWKADRAGNVIFRKSARNFNLPMCKAAETTVVEVEEIVDIGAFAPEDIHIPQIYVHRLIKGEKYEKRIERLSIRKEGDGEAKSAKPGDDVRERIIKRAALEFEDGMYANLGIGIPLLASNFISPNITVHLQSENGVLGLGPYPRQHEADADLINAGKETVTILPGASFFSSDESFAMIRGGHVDLTMLGAMQVSKYGDLANWMIPGKMVKGMGGAMDLVSSAKTKVVVTMEHSAKGNAHKIMEKCTLPLTGKQCVNRIITEKAVFDVDKKKGLTLIELWEGLTVDDVQKSTGCDFAVSPKLMPMQQIAN Predict reactive species Full Name: 3-oxoacid CoA transferase 1 Calculated Molecular Weight: 520 aa, 56 kDa Observed Molecular Weight: 56 kDa GenBank Accession Number: BC009001 Gene Symbol: OXCT1 Gene ID (NCBI): 5019 RRID: AB_2918924 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P55809 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.