Proteintech, 81078-6-RR, HVEM/TNFRSF14 Recombinant monoclonal antibody

Size: 20ul / 100ul The HVEM/TNFRSF14 (81078-6-RR) by Proteintech is a Recombinant antibody targeting HVEM/TNFRSF14 in WB, IHC, ELISA applications with reactivity to human samples 81078-6-RR targets HVEM/TNFRSF14 in WB, IHC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: human urine Positive IHC detected in: human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:500-1:2000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information The mammalian tumor necrosis factor receptor (TNFR) family consists of 10 cell-surface proteins that regulate development and homeostasis of the immune system. Member 14 of TNFR (TNFRSF14, also named as TR2, ATAR, HVEA, HVEM, LIGHTR) was also identified as a mediator of herpesvirus entry into mammalian cells. The cytoplasmic region of TNFRSF14 bound to several members of the TNFR-associated factor (TRAF) family, namely TRAF1, TRAF2, TRAF3, and TRAF5. Transient transfection into human 293 cells caused marked activation of nuclear factor- B (NF- B), a transcriptional regulator of multiple immunomodulatory and inflammatory genes. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Eg1275 Product name: recombinant human TNFRSF14 protein Source: mammalian cells -derived, pHZ-KIsec-C-6*HIS Tag: C-6*HIS Domain: 39-202 aa of BC002794 Sequence: LPSCKEDEYPVGSECCPKCSPGYRVKEACGELTGTVCEPCPPGTYIAHLNGLSKCLQCQMCDPAMGLRASRNCSRTENAVCGCSPGHFCIVQDGDHCAACRAYATSSPGQRVQKGGTESQDTLCQNCPPGTFSPNGTLEECQHQTKCSWLVTKAGAGTSSSHWV Predict reactive species Full Name: tumor necrosis factor receptor superfamily, member 14 (herpesvirus entry mediator) Calculated Molecular Weight: 30 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC002794 Gene Symbol: TNFRSF14 Gene ID (NCBI): 8764 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q92956 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.