Proteintech, 81219-2-RR, Phospho-SHP2 (Tyr580) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-SHP2 (Tyr580) (81219-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-SHP2 (Tyr580) in WB, ELISA applications with reactivity to human, mouse, rat samples 81219-2-RR targets Phospho-SHP2 (Tyr580) in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: PDGF treated NIH/3T3 cells, PDGF treated C6 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information Shp2, encoded by the PTPN11 gene, is a protein tyrosine phosphatase that acts as a positive regulator of Ras-Erk pathway. Shp2 contains two tandem Src homology 2 (SH2) domains, a protein tyrosine phosphatase domain and tyrosine phosphorylation sites. It has been suggested that the phosphorylated Tyr542 and Tyr580 recruit Grb2/Sos complex to activate Ras-Erk signaling. Recently, it was reported that ZAP70, a non-receptor tyrosine kinase, enhances phosphorylation of Shp2 at Tyr580 and activates Erk. (PMID: 23318428) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: protein tyrosine phosphatase, non-receptor type 11 Calculated Molecular Weight: 597 aa, 68 kDa Observed Molecular Weight: 72 kDa GenBank Accession Number: BC008692 Gene Symbol: PTPN11 Gene ID (NCBI): 5781 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q06124 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.