Proteintech, 81475-1-RR, Phospho-RPS6KA1 (Thr359/Ser363) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-RPS6KA1 (Thr359/Ser363) (81475-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-RPS6KA1 (Thr359/Ser363) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 81475-1-RR targets Phospho-RPS6KA1 (Thr359/Ser363) in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells Positive FC (Intra) detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension Background Information The 90 kDa ribosomal S6 kinases (RSK1-4) are a family of widely expressed serine/threonine kinases and play an important role in the MAPK signaling cascade and act downstream of ERK1/2 signaling. It can mediate mitogenic and stress-induced activation of the transcription factors CREB1, ETV1/ER81 and NR4A1/NUR77. RSK1 (RPS6KA1) is also designated as p90RSK associated with cellular proliferation, survival and differentiation. RPS6KA1 has some phosphorylation sites including Ser380, Thr359 and Ser363 which are important for kinase activation. (PMID: 16458888, 30813401, 17259979, 10679322) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ribosomal protein S6 kinase, 90kDa, polypeptide 1 Calculated Molecular Weight: 735 aa, 83 kDa Observed Molecular Weight: 83-90 kDa GenBank Accession Number: BC014966 Gene Symbol: RPS6KA1 Gene ID (NCBI): 6195 RRID: AB_2935552 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15418 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.