Proteintech, 81491-1-RR, Phospho-PDH E1 Alpha (Ser232) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-PDH E1 Alpha (Ser232) (81491-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-PDH E1 Alpha (Ser232) in WB, FC (Intra), ELISA applications with reactivity to human samples 81491-1-RR targets Phospho-PDH E1 Alpha (Ser232) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, λ phosphatase HEK-293T cells Positive FC (Intra) detected in: λ phosphatase treated HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension Background Information PDH E1 Alpha (PDHA1), as the major component of PDH, can be phosphorylated and inactivated by PDHK1. PDHA1 contains three serine residues (Ser232, Ser293, or Ser300) that can be reversibly phosphorylated by a dedicated family of four inhibitory pyruvate dehydrogenase kinases (PDHK1-4) and two reactivating phosphatases (PDP1, 2). Hypoxia induces the expression of PDHK1 and PDHK3 and hyperphosphorylates PDHA1. Phosphorylated PDHA1 inactivates the whole pyruvate dehydrogenase complex (PDC), reduces pyruvate entering into the tricarboxylic acid (TCA) cycle for oxidative phosphorylation, enhances the Warburg effect and promotes tumorigenesis. (PMID: 30993888, PMID: 34749809) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: pyruvate dehydrogenase (lipoamide) alpha 1 Calculated Molecular Weight: 43 kDa Observed Molecular Weight: 40 kDa GenBank Accession Number: BC002406 Gene Symbol: PDHA1 Gene ID (NCBI): 5160 RRID: AB_2935428 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P08559 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.