Proteintech, 81497-4-RR, HDAC2 Recombinant monoclonal antibody

Size: 20ul / 100ul The HDAC2 (81497-4-RR) by Proteintech is a Recombinant antibody targeting HDAC2 in WB, IHC, IF/ICC, IP, ELISA applications with reactivity to human, mouse samples 81497-4-RR targets HDAC2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, HEK-293 cells, K-562 cells, Jurkat cells, NIH/3T3 cells Positive IP detected in: HEK-293 cells Positive IHC detected in: mouse brain tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information Histone deacetylases(HDAC) are a class of enzymes that remove the acetyl groups from the lysine residues, leading to the formation of a condensed and transcriptionally silenced chromatin. Histone deacetylases act via the formation of large multiprotein complexes, and are responsible for the deacetylation of lysine residues at the N-terminal regions of core histones (H2A, H2B, H3, and H4). At least 4 classes of HDAC were identified. As a class, I HDAC, HDAC2 was primarily found in the nucleus. HDAC2 forms transcriptional repressor complexes by associating with many different proteins, including YY1, a mammalian zinc-finger transcription factor. Thus, it plays an important role in transcriptional regulation, cell cycle progression, and developmental events. This antibody is a rabbit monoclonal antibody raised against residues near the C terminus of human HDAC2. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3607 Product name: Recombinant human HDAC2 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 274-571 aa of BC031055 Sequence: TDRVMTVSFHKYGEYFPGTGDLRDIGAGKGKYYAVNFPMRDGIDDESYGQIFKPIISKVMEMYQPSAVVLQCGADSLSGDRLGCFNLTVKGHAKCVEVVKTFNLPLLMLGGGGYTIRNVARCWTHETAVALDCEIPNELPYNDYFEYFGPDFKLHISPSNMTNQNTPEYMEKIKQRLFENLRMLPHAPGVQMQAIPEDAVHEDSGDEDGEDPDKRISIRASDKRIACDEEFSDSEDEGEGGRRNVADHKKGAKKARIEEDKKETEDKKTDVKEEDKSKDNSGEKTDTKGTKSEQLSNP Predict reactive species Full Name: histone deacetylase 2 Calculated Molecular Weight: 458 aa, 52 kDa; 488 aa,55 kDa Observed Molecular Weight: 55-60 kDa GenBank Accession Number: BC031055 Gene Symbol: HDAC2 Gene ID (NCBI): 3066 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q92769 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.