Proteintech, 81525-1-RR, CD206 Recombinant monoclonal antibody

Size: 20ul / 100ul The CD206 (81525-1-RR) by Proteintech is a Recombinant antibody targeting CD206 in WB, IHC, IF-P, ELISA applications with reactivity to human, rat, pig samples 81525-1-RR targets CD206 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, rat, pig samples. Tested Applications Positive WB detected in: pig liver tissue, rat liver tissue, human placenta tissue Positive IHC detected in: human lung cancer tissue, human colon cancer tissue, human liver tissue, human lung tissue, human lymphoma tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF-P detected in: human placenta tissue Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Immunofluorescence (IF)-P: IF-P : 1:50-1:500 Background Information CD206, also named as MMR, CLEC13D and MRC1, is a type I membrane receptor that mediates the endocytosis of glycoproteins by macrophages. CD206 has been shown to bind high-mannose structures on the surface of potentially pathogenic viruses, bacteria, and fungi so that they can be neutralized by phagocytic engulfment. CD206 is a 170 kDa transmembrane protein which contains 5 domains: an amino-terminal cysteine-rich region, a fibronectin type II repeat, a series of eight tandem lectin-like carbohydrate recognition domains (responsible for the recognition of mannose and fucose), a transmembrane domain, and an intracellular carboxy-terminal tail. It is expressed on most tissue macrophages, in vitro derived dendritic cells, lymphatic and sinusoidal endothelia. Specification Tested Reactivity: human, rat, pig Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: mannose receptor, C type 1 Calculated Molecular Weight: 166 kDa Observed Molecular Weight: 170 kDa GenBank Accession Number: NM_002438 Gene Symbol: CD206 Gene ID (NCBI): 4360 RRID: AB_2935558 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P22897 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.