Proteintech, 81592-1-RR, Phospho-p70(S6K) (Thr229)/p85(S6K) (Thr252) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-p70(S6K) (Thr229)/p85(S6K) (Thr252) (81592-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-p70(S6K) (Thr229)/p85(S6K) (Thr252) in WB, ELISA applications with reactivity to human samples 81592-1-RR targets Phospho-p70(S6K) (Thr229)/p85(S6K) (Thr252) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: IGF-1 treated MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Background Information RPS6KB1(Ribosomal protein S6 kinase beta-1) is also named as STK14A, p70 S6KA, and belongs to the S6 kinase subfamily. RPS6KB1 is a major substrate of mTOR and acts as a crucial effector of mTOR signaling pathway. It plays a key role in cell growth and proliferation by regulating INS sensitivity, metabolism, protein synthesis, and cell cycle. RPS6KB1 may play an important role in the progression of HCC and could serve as a potential molecular target for HCC therapy (PMID:22684641). The Rps6kb1 gene encodes the 70 kDa ribosomal protein S6 kinase (p70S6K). The PI3K/mTOR signalling pathway is one of the major mechanisms for controlling cell survival, proliferation, and metabolism and is the central regulator of translation of some components of the protein synthesis system. Due to alternative translation, two isoform S6K1 proteins are known to exist in mammalian cells: p85 S6K1 and p70 S6K1, which is identical to p85 S6K but lacks its first 23 amino acids. In addition, mammalian cells express a second S6K1 isoform spanning 316 amino acids (p31 S6K1). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: ribosomal protein S6 kinase, 70kDa, polypeptide 1 Calculated Molecular Weight: 59 kDa Observed Molecular Weight: 65-85 kDa GenBank Accession Number: BC053365 Gene Symbol: p70 S6K Gene ID (NCBI): 6198 RRID: AB_2935429 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P23443 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.