Proteintech, 81611-1-RR, ECHS1 Recombinant monoclonal antibody

Size: 20ul / 100ul The ECHS1 (81611-1-RR) by Proteintech is a Recombinant antibody targeting ECHS1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 81611-1-RR targets ECHS1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HeLa cells, MCF-7 cells, mouse liver tissue, L02 cells, Caco-2 cells, HepG2 cells, HEK-293 cells, PC-3 cells, rat liver tissue Positive IHC detected in: human prostate cancer tissue, human colon tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Background Information Enoyl-coenzyme A hydratase (ECHS1) is a mitochondrial protein which catalyzes the hydration of 2-trans-enoyl-coenzyme A intermediates to L-3-hydroxyacyl-coenzyme A, playing a key role in metabolism of fatty acids in mitochondria. ECHS1 is highly expressed in muscle, liver and fibroblasts. Altered expression of ECHS1 has been considered a characteristic feature of mitochondria dysfunction. (23275097, 23235493) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1842 Product name: Recombinant human ECHS1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-290 aa of BC008906 Sequence: MAALRVLLSCVRGPLRPPVRCPAWRPFASGANFEYIIAEKRGKNNTVGLIQLNRPKALNALCDGLIDELNQALKIFEEDPAVGAIVLTGGDKAFAAGADIKEMQNLSFQDCYSSKFLKHWDHLTQVKKPVIAAVNGYAFGGGCELAMMCDIIYAGEKAQFAQPEILIGTIPGAGGTQRLTRAVGKSLAMEMVLTGDRISAQDAKQAGLVSKICPVETLVEEAIQCAEKIASNSKIVVAMAKESVNAAFEMTLTEGSKLEKKLFYSTFATDDRKEGMTAFVEKRKANFKDQ Predict reactive species Full Name: enoyl Coenzyme A hydratase, short chain, 1, mitochondrial Calculated Molecular Weight: 31 kDa Observed Molecular Weight: 30 kDa GenBank Accession Number: BC008906 Gene Symbol: ECHS1 Gene ID (NCBI): 1892 RRID: AB_2935566 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P30084 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.