Proteintech, 81740-1-RR, Phospho-CHEK2 (Thr68) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-CHEK2 (Thr68) (81740-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-CHEK2 (Thr68) in WB, FC (Intra), ELISA applications with reactivity to human samples 81740-1-RR targets Phospho-CHEK2 (Thr68) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: MMS treated PC-3 cells, UV treated HeLa cells Positive FC (Intra) detected in: MMS treated PC-3 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.06 ug per 10^6 cells in a 100 µl suspension Background Information Serine/threonine-protein kinase Chk2 (CHEK2) is a serine/threonine kinase which is activated upon DNA damage and is implicated in pathways that govern DNA repair, cell cycle arrest or apoptosis in response to the initial damage. ATM phosphorylates CHEK2 on T68. Phosphorylation on T68 and subsequent full activation of CHEK2 was shown to require priming phosphorylation on adjacent residues by Polo-like kinase 3 (PLK3) and the dualspecificity tyrosine and serine/threoninekinase TTK/hMPS1. Additionally TTK appears to phosphorylate T68. Phosphorylation of T68 promotes the binding of the N-terminal SQ/TQ-rich cluster of one CHEK2 molecule with the FHA domain of another CHEK2 molecule. (PMID: 28553140, PMID: 18004398, PMID: 33322746) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: CHK2 checkpoint homolog (S. pombe) Calculated Molecular Weight: 61 kDa Observed Molecular Weight: 65 kDa GenBank Accession Number: BC004207 Gene Symbol: CHEK2 Gene ID (NCBI): 11200 RRID: AB_2935575 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: O96017 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.