Proteintech, 81767-2-RR, Phospho-ZAP70 (Tyr319)/SYK (Tyr352) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-ZAP70 (Tyr319)/SYK (Tyr352) (81767-2-RR) by Proteintech is a Recombinant antibody targeting Phospho-ZAP70 (Tyr319)/SYK (Tyr352) in WB, ELISA applications with reactivity to human samples 81767-2-RR targets Phospho-ZAP70 (Tyr319)/SYK (Tyr352) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: H2O2 treated Jurkat cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Zeta-associated protein (ZAP)-70, a member of the Syk family of tyrosine kinases, plays an important role in T-cell receptor signaling, natural killer (NK) cell activation and early B-cell development. Three tyrosine residues (Tyr292, Tyr315 and Tyr319) in the interdomain B region of ZAP‐70 have attracted particular attention as sites that might regulate ZAP‐70 activity and/or binding to heterologous proteins. The third candidate phosphorylation site in the interdomain B region of ZAP‐70 is Tyr319. Although the functional importance of this site in ZAP‐70 is unknown, a previous report presented indirect evidence that the orthologous Tyr352 residue in Syk is involved in the association of PLC‐γ1 with this PTK. (PMID: 10202147) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: zeta-chain (TCR) associated protein kinase 70kDa Calculated Molecular Weight: 70 kDa Observed Molecular Weight: 70 kDa, 72kDa GenBank Accession Number: BC039039 Gene Symbol: ZAP70 Gene ID (NCBI): 7535 ENSEMBL Gene ID: ENSG00000115085 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P43403 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.