Proteintech, 81802-2-RR, NESP55,GNAS Recombinant monoclonal antibody

Size: 20ul / 100ul The NESP55,GNAS (81802-2-RR) by Proteintech is a Recombinant antibody targeting NESP55,GNAS in WB, IF/ICC, ELISA applications with reactivity to human, mouse, rat samples 81802-2-RR targets NESP55,GNAS in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: SH-SY5Y cells, Jurkat cells, MCGF-7 cells, mouse liver tissue, rat liver tissue Positive IF/ICC detected in: MCF-7 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The near two bands represent two different isoforms. The expression profile/molecular weight observed are consistent with what has been described in the literature (PMID : 3258290). Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0197 Product name: Recombinant human NESP55,GNAS protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 230-394 aa of BC002722 Sequence: ERRKWIQCFNDVTAIIFVVASSSYNMVIREDNQTNRLQEALNLFKSIWNNRWLRTISVILFLNKQDLLAEKVLAGKSKIEDYFPEFARYTTPEDATPEPGEDPRVTRAKYFIRDEFLRISTASGDGRHYCYPHFTCAVDTENIRRVFNDCRDIIQRMHLRQYELL Predict reactive species Full Name: GNAS complex locus Calculated Molecular Weight: 45 kDa Observed Molecular Weight: 46 kDa GenBank Accession Number: BC002722 Gene Symbol: GNAS Gene ID (NCBI): 2778 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P63092 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.