Proteintech, 81812-4-RR, Phospho-4EBP1 (Thr37) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-4EBP1 (Thr37) (81812-4-RR) by Proteintech is a Recombinant antibody targeting Phospho-4EBP1 (Thr37) in WB, ELISA applications with reactivity to human samples 81812-4-RR targets Phospho-4EBP1 (Thr37) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293T cells, λ phosphatase treated HEK-293T cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Background Information 4EBP1 (4E-BP1) is a translational regulator and functions by sequestering eukaryotic translation initiation factor 4E (eIF4E) from the translation initiation machinery. 4E-BP1 contains at least six phosphorylation sites (Thr37, Thr46, Ser65, Thr70, Ser83, and Ser112), four of which (Thr37, Thr46, Ser65, and Thr70) are known to be regulated by mTOR signaling. Phosphorylation of Thr37 and Thr46 is thought to prime 4E-BP1 for sequential phosphorylation of Ser65 and Thr70, which results in the dissociation of 4E-BP1 from eIF4E. (PMID: 12747827) Specification Tested Reactivity: human Cited Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: eukaryotic translation initiation factor 4E binding protein 1 Calculated Molecular Weight: 118 aa, 12 kDa Observed Molecular Weight: 15-20 kDa GenBank Accession Number: BC004459 Gene Symbol: EIF4EBP1 Gene ID (NCBI): 1978 RRID: AB_3670507 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q13541 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.