Proteintech, 82335-1-RR, Phospho-NF-κB p65 (Ser468) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-NF-κB p65 (Ser468) (82335-1-RR) by Proteintech is a Recombinant antibody targeting Phospho-NF-κB p65 (Ser468) in WB, FC (Intra), ELISA applications with reactivity to human, mouse samples 82335-1-RR targets Phospho-NF-κB p65 (Ser468) in WB, IHC, IF, FC (Intra), ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Calyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells Positive FC (Intra) detected in: Calyculin A treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information Nuclear factor kB (NF-kB) is a collective term for a small family of dimeric transcription factors [comprising p65 (RelA) and RelB, c-Rel, p50/p105 (NF-κB1), and p52/p100 (NF-κB2)]. All NF-κB proteins share a Rel homology domain (RHD), which is responsible for DNA binding and dimerization. Only p65, RelB, and c-Rel contain potent transactivation domains within sequences from the C-terminal to the RHD. Exterior signals lead to the phosphorylation and degradation of the inhibitory complex IκB, which is modulated by the IκB kinase (IKK), and its degradation allows for the release of the typical NF-κB heterodimer, p65/p50, to translocate into the nucleus. NF-κB binds to its cognate DNA elements and can transcriptionally activate different target genes among which 200-500 genes have been implicated in cell survival/apoptosis, cell growth, immune response, and inflammation. Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse, rat, pig, chicken Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: v-rel reticuloendotheliosis viral oncogene homolog A (avian) Calculated Molecular Weight: 65 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC011603 Gene Symbol: NF-κB p65 Gene ID (NCBI): 5970 RRID: AB_3083091 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q04206 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.