Proteintech, 82508-1-RR, FH Recombinant monoclonal antibody

Size: 20ul / 100ul The FH (82508-1-RR) by Proteintech is a Recombinant antibody targeting FH in WB, IHC, IP, ELISA applications with reactivity to human, mouse, rat samples 82508-1-RR targets FH in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: HepG2 cells, HEK-293 cells, A431 cells, L02 cells, mouse liver tissue, rat liver tissue Positive IP detected in: mouse liver tissue Positive IHC detected in: human stomach tissue, human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information FH(fumarate hydratase) belongs to the class-II fumarase/aspartase family. It is an enzymatic component of the tricarboxylic acid cycle, catalyzing the conversion of fumarate to malate. FH also acts as a tumor suppressor function of FH in sporadic kidney cancer(PMID:21695080). Mutations in this gene can cause fumarase deficiency and lead to progressive encephalopathy. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag1934 Product name: Recombinant human FH protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 204-510 aa of BC003108 Sequence: HEVLLPGLQKLHDALDAKSKEFAQIIKIGRTHTQDAVPLTLGQEFSGYVQQVKYAMTRIKAAMPRIYELAAGGTAVGTGLNTRIGFAEKVAAKVAALTGLPFVTAPNKFEALAAHDALVELSGAMNTTACSLMKIANDIRFLGSGPRSGLGELILPENEPGSSIMPGKVNPTQCEAMTMVAAQVMGNHVAVTVGGSNGHFELNVFKPMMIKNVLHSARLLGDASVSFTENCVVGIQANTERINKLMNESLMLVTALNPHIGYDKAAKIAKTAHKNGSTLKETAIELGYLTAEQFDEWVKPKDMLGPK Predict reactive species Full Name: fumarate hydratase Calculated Molecular Weight: 510 aa, 55 kDa Observed Molecular Weight: 45 kDa GenBank Accession Number: BC003108 Gene Symbol: FH Gene ID (NCBI): 2271 RRID: AB_3086485 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P07954 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.