Proteintech, 82677-1-RR, MX1 Recombinant monoclonal antibody

Size: 20ul / 100ul The MX1 (82677-1-RR) by Proteintech is a Recombinant antibody targeting MX1 in WB, IHC, IF/ICC, ELISA applications with reactivity to human samples 82677-1-RR targets MX1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: IFN alpha treated A549 cells, IFN alpha treated HepG2 cells Positive IHC detected in: human lung cancer tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: A549 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Background Information Mx1 (also known as MxA) is a GTPase that belongs to the Mx family of proteins, which are ubiquitous in eukaryotes and involved in the immune response to viral infections. The human Mx1 is localized in the cytoplasm interfering with the translation of viral proteins, whereas the mouse Mx1 accumulates in the nucleus and inhibits the primary transcription of viral RNA. Mx1 expression is induced by treatment with α/β IFN. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag4692 Product name: Recombinant human MX1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 312-662 aa of BC032602 Sequence: LLEEGKATVPCLAEKLTSELITHICKSLPLLENQIKETHQRITEELQKYGVDIPEDENEKMFFLIDKINAFNQDITALMQGEETVGEEDIRLFTRLRHEFHKWSTIIENNFQEGHKILSRKIQKFENQYRGRELPGFVNYRTFETIVKQQIKALEEPAVDMLHTVTDMVRLAFTDVSIKNFEEFFNLHRTAKSKIEDIRAEQEREGEKLIRLHFQMEQIVYCQDQVYRGALQKVREKELEEEKKKKSWDFGAFQSSSATDSSMEEIFQHLMAYHQEASKRISSHIPLIIQFFMLQTYGQQLQKAMLQLLQDKDTYSWLLKERSDTSDKRKFLKERLARLTQARRRLAQFPG Predict reactive species Full Name: Myxovirus resistance protein 1 Calculated Molecular Weight: 662 aa, 76 kDa Observed Molecular Weight: 75 kDa GenBank Accession Number: BC032602 Gene Symbol: MX1 Gene ID (NCBI): 4599 RRID: AB_3086503 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P20591 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.