Proteintech, 82707-13-RR, Cleaved Caspase 3/P17/P19 Recombinant monoclonal antibody

Size: 20ul / 100ul The Cleaved Caspase 3/P17/P19 (82707-13-RR) by Proteintech is a Recombinant antibody targeting Cleaved Caspase 3/P17/P19 in WB, IHC, ELISA applications with reactivity to human, mouse samples 82707-13-RR targets Cleaved Caspase 3/P17/P19 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Staurosporine treated Jurkat cells Positive IHC detected in: Jurkat cellsNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:1000-1:4000 Background Information Caspases, a family of endoproteases, are critical players in cell regulatory networks controlling inflammation and cell death. Initiator caspases (caspase-2, -8, -9, -10, -11, and -12) cleave and activate downstream effector caspases (caspase-3, -6, and -7), which in turn execute apoptosis by cleaving targeted cellular proteins. Caspase 3 plays a key role in the activation of sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Caspase 3 can form heterocomplex with other proteins. This antibody can recognize p17 and p19 fragment of Caspase 3. This antibody is specific for cleaved caspase 3, and does not recognize full length caspase-3. The cleaved P17 and P19 fragment might form complex and shows at around 30-35 kDa by western blot (PMID: 25501826). Specification Tested Reactivity: human, mouse Cited Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: caspase 3, apoptosis-related cysteine peptidase Calculated Molecular Weight: 32 kDa Observed Molecular Weight: 17 kDa, 19 kDa GenBank Accession Number: NM_032991 Gene Symbol: Caspase 3 Gene ID (NCBI): 836 RRID: AB_3670535 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P42574 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.