Proteintech, 82734-4-RR, SARS-CoV-2 Spike Recombinant monoclonal antibody

Size: 20ul / 100ul The SARS-CoV-2 Spike (82734-4-RR) by Proteintech is a Recombinant antibody targeting SARS-CoV-2 Spike in WB, ELISA applications with reactivity to virus samples 82734-4-RR targets SARS-CoV-2 Spike in WB, ELISA applications and shows reactivity with virus samples. Tested Applications Positive WB detected in: Recombinant protein Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Background Information Coronaviruses (CoVs) infect human and animals and cause varieties of diseases, including respiratory, enteric, renal, and neurological diseases. CoV uses its spike protein to recognize ACE2 as its receptors and mediate membrane fusion and virus entry into host cells(PMID: 32221306). Each monomer of trimeric S protein is about 180 kDa, and contains two subunits, S1 and S2,S1 recognizes and binds to host receptors, and subsequent conformational changes in S2 facilitate fusion between the viral envelope and the host cell membrane(PMID: 19198616). Although the amino acid sequences of the S-glycoprotein were found to be different between the various HCoV, the structures showed high similarity, but the best 3D structural overlap shared by SARS-CoV and SARS-CoV-2, consistent with the shared ACE2 predicted receptor (PMID: 32522207). The spike protein of CoVs can be a target for vaccine and therapeutic development (PMID: 19198616). 28901-1-AP is specific for spike protein of SARS-COV-2, that antigen region is 428-506aa. Specification Tested Reactivity: virus Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag30684 Product name: Recombinant virus spike protein protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 428-506 aa of NC_045512 Sequence: DFTGCVIAWN SNNLDSKVGG NYNYLYRLFR KSNLKPFERD ISTEIYQAGS TPCNGVEGFN CYFPLQSYGF QPTNGVGYQ Predict reactive species Full Name: SARS-CoV-2 Spike Protein Calculated Molecular Weight: 141 kDa GenBank Accession Number: NC_045512 Gene Symbol: SARS-COV-2 Gene ID (NCBI): 43740568 RRID: AB_3670542 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.