Proteintech, 82736-11-RR, Phospho-EGFR (Tyr1016) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-EGFR (Tyr1016) (82736-11-RR) by Proteintech is a Recombinant antibody targeting Phospho-EGFR (Tyr1016) in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 82736-11-RR targets Phospho-EGFR (Tyr1016) in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: EGF treated A431 cells Positive IF/ICC detected in: EGF treated A431 cells Positive FC (Intra) detected in: EGF treated A431 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Epidermal Growth Factor Receptor (EGFR) is one of the receptor tyrosine kinases (RTKs), members of the ErbB/HER family which consists of ErbB1 (EGFR or HER1), ErbB2 (HER2 or Neu), ErbB3 (HER3), and ErbB4 (HER4). Phosphorylation at Tyr1016 is essential for the recruitment of downstream signaling molecules. For example, it can interact with adapter proteins like GRB2, which in turn activates the Ras-MAPK pathway, leading to cell proliferation and differentiation. (PMID: 26628682) Some scientists call EGFR Tyr1016 as EGFR Tyr992, the site where the signaling peptide is removed. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: epidermal growth factor receptor (erythroblastic leukemia viral (v-erb-b) oncogene homolog, avian) Calculated Molecular Weight: 1210 aa, 134 kDa Observed Molecular Weight: 170 kDa GenBank Accession Number: BC094761 Gene Symbol: EGFR Gene ID (NCBI): 1956 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P00533 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.