Proteintech, 82751-5-RR, Phospho-CDK1 (Tyr15) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-CDK1 (Tyr15) (82751-5-RR) by Proteintech is a Recombinant antibody targeting Phospho-CDK1 (Tyr15) in WB, FC (Intra), ELISA applications with reactivity to human samples 82751-5-RR targets Phospho-CDK1 (Tyr15) in WB, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, λ phosphatase treated HeLa cells Positive FC (Intra) detected in: λ phosphatase treated HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.13 ug per 10^6 cells in a 100 µl suspension Background Information CDK1, also named as CDC2, belongs to the protein kinase superfamily, CMGC Ser/Thr protein kinase family and CDC2/CDKX subfamily. Phospho-CDK1 (Tyr15) is the inactive, inhibited form of cyclin-dependent kinase 1 (CDK1) generated when the tyrosine 15 residue of CDK1's ATP-binding loop is phosphorylated by the kinases WEE1 and PKMYT1. This single phosphorylation event clamps the kinase in an inactive conformation, preventing premature onset of mitosis by blocking ATP binding and cyclin B1 activation. (PMID: 14645578) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: cell division cycle 2, G1 to S and G2 to M Calculated Molecular Weight: 25 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC014563 Gene Symbol: CDK1 Gene ID (NCBI): 983 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P06493 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.