Proteintech, 82816-4-RR, Phospho-AXL (Tyr779) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-AXL (Tyr779) (82816-4-RR) by Proteintech is a Recombinant antibody targeting Phospho-AXL (Tyr779) in WB, ELISA applications with reactivity to human samples 82816-4-RR targets Phospho-AXL (Tyr779) in WB, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: pervanadate treated A172 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:6000 Background Information Axl, also known as Ufo or Ark, is a receptor tyrosine kinase of the TAM (Tyro3, Axl and Mer) family. Phospho-AXL (Tyr779) refers to the phosphorylated form of the receptor tyrosine kinase AXL at tyrosine 779 within its intracellular kinase domain. Upon ligand binding (e.g., Gas6 or HGF), AXL dimerizes and undergoes trans-autophosphorylation, generating phospho-Tyr779 that serves as a docking site for the p85 regulatory subunit of PI3K, thereby activating the PI3K/AKT/mTOR pathway and promoting cell survival, migration, and invasion. (PMID: 28251492) Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: AXL receptor tyrosine kinase Calculated Molecular Weight: 894 aa, 98 kDa Observed Molecular Weight: 140 kDa GenBank Accession Number: BC032229 Gene Symbol: AXL Gene ID (NCBI): 558 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P30530 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.