Proteintech, 82827-1-RR, ICAM-1/CD54 Recombinant monoclonal antibody

Size: 20ul / 100ul The ICAM-1/CD54 (82827-1-RR) by Proteintech is a Recombinant antibody targeting ICAM-1/CD54 in WB, IHC, ELISA applications with reactivity to mouse, rat samples 82827-1-RR targets ICAM-1/CD54 in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples. Tested Applications Positive WB detected in: mouse lung tissue, mouse spleen tissue, rat lung tissue Positive IHC detected in: mouse lung tissue, mouse liver tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Background Information ICAM-1 (CD54) is a transmembrane glycoprotein of the immunoglobulin superfamily and is critical for the firm attachment and transmigration of leukocytes out of blood vessels and into tissues (PMID: 19307690). ICAM-1 is expressed by several cell types, typically on endothelial cells and cells of the immune system, and its expression can be up-regulated by various stimuli, including TNF-α, INF-γ, IL-1 and thrombin (PMID: 3086451; 9694714; 15979056). It is a ligand for LFA-1 and Mac-1, serves as a receptor for rhinovirus, and is one of several receptors used by Plasmodium falciparum (PMID: 2566624; 2538244; 2475784). Specification Tested Reactivity: mouse, rat Cited Reactivity: mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Recombinant protein Predict reactive species Full Name: intercellular adhesion molecule 1 Calculated Molecular Weight: 59 kDa Observed Molecular Weight: 85-110 kDa GenBank Accession Number: NM_010493 Gene Symbol: ICAM-1 Gene ID (NCBI): 15894 RRID: AB_3670566 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P13597 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.