Proteintech, 82828-10-RR, Phospho-Histone H3 (Ser10) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Histone H3 (Ser10) (82828-10-RR) by Proteintech is a Recombinant antibody targeting Phospho-Histone H3 (Ser10) in WB, IHC, IF/ICC, ELISA, ChIP-qPCR applications with reactivity to human, mouse, rat samples 82828-10-RR targets Phospho-Histone H3 (Ser10) in WB, IHC, IF/ICC, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: nocodazole treated HeLa cells, Calyculin A treated HSC-T6 cells, nocodazole NIH/3T3 cells Positive IHC detected in: mouse brain tissue, human colon cancer tissue, human ovary cancer tissue, human tonsillitis tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HepG2 cells Positive ChIP-qPCR detected in: Nocodazole (100 ng/ml, 16 h) HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:500-1:2000 Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information Phospho-histone-H3 (PHH3) is a core histone protein, which in its phosphorylated state forms the principal constituents of eukaryotic chromatin, with histone H3 being phosphorylated at serine (Ser) 10 or Ser28 as well as its phosphorylation of Ser10 being strongly correlated with the late G2 to M-phase transition in mammalian mitotic cells. On the basis of previous research, a few cell line- and animal model-based researches have displayed an increase in phosphorylation of histone H3 at Ser10 (H3S10ph), the only histone marker that is involved in carcinogenesis and cellular transformation. Histone H3 phosphorylation on serine-10 is specific to mitosis and phosphorylated histone H3 (PHH3) proliferation markers (as counts defined per area or as indices defined per cell numbers) are increasingly being used to evaluate proliferation in various tumors. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H3a Calculated Molecular Weight: 15 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: NM_003529 Gene Symbol: HIST1H3A Gene ID (NCBI): 8350 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P68431 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.