Proteintech, 82830-15-RR, Phospho-INSR (Tyr1146)/IGF1R (Tyr1131) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-INSR (Tyr1146)/IGF1R (Tyr1131) (82830-15-RR) by Proteintech is a Recombinant antibody targeting Phospho-INSR (Tyr1146)/IGF1R (Tyr1131) in WB, ELISA applications with reactivity to human, mouse, rat samples 82830-15-RR targets Phospho-INSR (Tyr1146)/IGF1R (Tyr1131) in WB, ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: IGF-1 treated HEK-293 cells, IGF-1 treated C6 cells, IGF-1 treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Background Information Insulin binding to the insulin receptor (INSR) triggers sequential conformational changes and autophosphorylation of the receptor, followed by activation of a kinase signaling cascade that plays essential roles in a wide variety of biological processes. INSR belongs to a class of receptor tyrosine kinases (RTKs) that comprises 58 receptors in humans. The INSR shares a high structural homology with the IGF1R (84% similarity in the tyrosine kinase domain, 45-65% in the ligand-binding domain, and more than 50% in the overall amino acid sequence). In addition, ligand-dependent activation of the INSR and IGF1R activates almost identical downstream signaling cascades. Insulin binds to INSR in peripheral tissues, initiating receptor activation followed by intracellular signaling cascades. The first step in INSR activation is the autophosphorylation of intracellular tyrosine residues in the JM domain, kinase activation loop, and CT domain. Phosphorylation of three tyrosine residues (Tyr1146, Tyr1150, and Tyr1151, based on INSR isoform A numbering) located in the kinase activation loop plays a crucial role in kinase activity regulation. Insulin binding also induces INSR kinase-mediated phosphorylation of four tyrosine residues located in the JM (Tyr953 and Tyr960) and CT domain (Tyr1316 and Tyr1322). (PMID: 37779149,PMID:24434591) Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: INSR Observed Molecular Weight: 95 kDa GenBank Accession Number: BC117172 Gene Symbol: INSR Gene ID (NCBI): 3643 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P06213 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.