Proteintech, 82835-3-RR, Phospho-EIF2AK4 (Thr899) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-EIF2AK4 (Thr899) (82835-3-RR) by Proteintech is a Recombinant antibody targeting Phospho-EIF2AK4 (Thr899) in WB, ELISA applications with reactivity to human, mouse samples 82835-3-RR targets Phospho-EIF2AK4 (Thr899) in WB, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Calyculin A treated HeLa cells, Calyculin A treated NIH/3T3 cells Recommended dilution Western Blot (WB): WB : 1:2000-1:14000 Background Information EIF2AK4 (eukaryotic translation initiation factor 2 alpha kinase 4) belongs to a family of protein kinases that phosphorylate the α subunit of EIF2 (eukaryotic translation initiation factor 2) in response to various stress stimuli. Phosphorylation of EIF2A in response to EIF2AK4 activation subsequently increases translation of specific mRNAs that are important for stress remediation such as ATF4 (activating transcription factor 4). (PMID: 26986695) Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: eukaryotic translation initiation factor 2 alpha kinase 4 Calculated Molecular Weight: 187 kDa Observed Molecular Weight: 187 kDa GenBank Accession Number: BC009350 Gene Symbol: EIF2AK4 Gene ID (NCBI): 440275 RRID: AB_3670570 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: Q9P2K8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.