Proteintech, 82869-1-RR, cGAS Recombinant monoclonal antibody

Size: 20ul / 100ul The cGAS (82869-1-RR) by Proteintech is a Recombinant antibody targeting cGAS in WB, IF/ICC, ELISA applications with reactivity to human samples 82869-1-RR targets cGAS in WB, IF/ICC, ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HAP1 cells, HL-60 cells, THP-1 cells, MCF-7 cells, NK-92 cells Positive IF/ICC detected in: A431 cells Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information cGAS (Cyclic GMP-AMP synthase), also known as C6orf150 or h-cGAS, is a 522 aa protein. cGAS mediates innate immune responses against invading pathogens, or against self-dsDNA, which causes autoimmune disorders. The cGAS sensor not only recognizes cytosolic dsDNA but also synthesizes the second messenger cGAMP from ATP and GTP, which then binds to and activates STING. STING undergoes conformational changes and translocation from the endoplasmic reticulum to the Golgi apparatus to encounter TBK1 and IRF3, eventually triggering the production of type I IFNs. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag23455 Product name: Recombinant human C6orf150 protein Source: e coli. -derived, PET30a Tag: 6*His Domain: 1-233 aa of BC113608 Sequence: MQPWHGKAMQRASEAGATAPKASARNARGAPMDPNESPAAPEAALPKAGKFGPARKSGSRQKKSAPDTQERPPVRATGARAKKAPQRAQDTQPSDATSAPGAEGLEPPAAREPALSRAGSCRQRGARCSTKPRPPPGPWDVPSPGLPVSAPILVRRDAAPGASKLRAVLEKLKLSRDDISTAAGMVKGVVDHLLLRLKCDSAFRGVGLLNTGSYYEHVKISAPNEFDVMFKLE Predict reactive species Full Name: chromosome 6 open reading frame 150 Calculated Molecular Weight: 496 aa, 54 kDa Observed Molecular Weight: 62 kDa GenBank Accession Number: BC113608 Gene Symbol: cGAS Gene ID (NCBI): 115004 RRID: AB_3670589 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8N884 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.