Proteintech, 82888-3-RR, Emerin Recombinant monoclonal antibody

Size: 20ul / 100ul The Emerin (82888-3-RR) by Proteintech is a Recombinant antibody targeting Emerin in WB, IHC, FC (Intra), ELISA applications with reactivity to human samples 82888-3-RR targets Emerin in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, Jurkat cells Positive IHC detected in: human stomach cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Immunohistochemistry (IHC): IHC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag0236 Product name: Recombinant human EMD protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 66-252 aa of BC000738 Sequence: STRGDADMYDLPKKEDALLYQSKGYNDDYYEESYFTTRTYGEPESAGPSRAVRQSVTSFPDADAFHHQVHDDDLLSSSEEECKDRERPMYGRDSAYQSITHYRPVSASRSSLDLSYYPTSSSTSFMSSSSSSSSWLTRRAIRPENRAPGAGLGQDRQVPLWGQLLLFLVFVIVLFFIYHFMQAEEGN Predict reactive species Full Name: emerin Calculated Molecular Weight: 34 kDa Observed Molecular Weight: 35 kDa GenBank Accession Number: BC000738 Gene Symbol: EMD Gene ID (NCBI): 2010 RRID: AB_3670610 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purfication UNIPROT ID: P50402 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.