Proteintech, 82961-1-RR, BBS4 Recombinant monoclonal antibody

Size: 20ul / 100ul The BBS4 (82961-1-RR) by Proteintech is a Recombinant antibody targeting BBS4 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 82961-1-RR targets BBS4 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HEK-293 cells, HeLa cells, SH-SY5Y cells Positive IF/ICC detected in: hTERT-RPE1 cells Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information BBS4 (Bardet-Biedl syndrome protein 4) is one of the early BBS proteins discovered, with 35 mutations reported to cause the BBS phenotype (PMID: 26762677). BBS4 undergoes localization to the centriolar satellites of centrosomes and basal body of primary cilia, where it takes part in recruiting cargo to centriolar satellites and allowing the formation of a functional centrosomal microtubule organizing center (MTOC) (PMID: 25860617). Some research has found that BBS4 expression in humans during the development of CNS and sensory organs, concerning the cardinal clinical signs of BBS (PMID: 33860840). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3430 Product name: Recombinant human BBS4 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-351 aa of BC027624 Sequence: MAEERVATRTQFPVSTESQKPRQKKAPEFPILEKQNWLIHLHYIRKDYEACKAVIKEQLQETQGLCEYAIYVQALIFRLEGNIQESLELFQTCAVLSPQSADNLKQVARSLFLLGKHKAAIEVYNEAAKLNQKDWEISHNLGVCYIYLKQFNKAQDQLHNALNLNRHDLTYIMLGKIHLLEGDLDKAIEVYKKAVEFSPENTELLTTLGLLYLQLGIYQKAFEHLGNALTYDPTNYKAILAAGSMMQTHGDFDVALTKYRVVACAVPESPPLWNNIGMCFFGKKKYVAAISCLKRANYLAPFDWKILYNLGLVHLTMQQYASAFHFLSAAINFQPKMGELYMLLAVALTNL Predict reactive species Full Name: Bardet-Biedl syndrome 4 Calculated Molecular Weight: 519 aa, 58 kDa Observed Molecular Weight: 60 kDa GenBank Accession Number: BC027624 Gene Symbol: BBS4 Gene ID (NCBI): 585 RRID: AB_3670707 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96RK4 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.