Proteintech, 82962-1-RR, SNIP1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SNIP1 (82962-1-RR) by Proteintech is a Recombinant antibody targeting SNIP1 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to Human samples 82962-1-RR targets SNIP1 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with Human samples. Tested Applications Positive WB detected in: HeLa cells, Jurkat cells, HT-29 cells, U2OS cells, BxPC-3 cells Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: U2OS cells Positive FC detected in: U2OS cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Smad nuclear-interacting protein 1 (SNIP1) is a novel interacting factor of the Smad protein family that is expressed in different cell types and is involved in cell proliferation, apoptosis, and differentiation. Specification Tested Reactivity: Human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag33543 Product name: Recombinant human SNIP1 protein Source: e coli. -derived, PET28a Tag: 6*His Domain: 1-396 aa of BC027040 Sequence: MKAVKSERERGSRRRHRDGDVVLPAGVVVKQERLSPEVAPPAHRRPDHSGGSPSPPTSEPARSGHRGNRARGVSRSPPKKKNKASGRRSKSPRSKRNRSPHHSTVKVKQEREDHPRRGREDRQHREPSEQEHRRARNSDRDRHRGHSHQRRTSNERPGSGQGQGRDRDTQNLQAQEEEREFYNARRREHRQRNDVGGGGSESQELVPRPGGNNKEKEVPAKEKPSFELSGALLEDTNTFRGVVIKYSEPPEARIPKKRWRLYPFKNDEVLPVMYIHRQSAYLLGRHRRIADIPIDHPSCSKQHAVFQYRLVEYTRADGTVGRRVKPYIIDLGSGNGTFLNNKRIEPQRYYELKEKDVLKFGFSSREYVLLHESSDTSEIDRKDDEDEEEEEEVSDS* Predict reactive species Full Name: Smad nuclear interacting protein 1 Calculated Molecular Weight: 46 kDa Observed Molecular Weight: 46-50 kDa GenBank Accession Number: BC027040 Gene Symbol: SNIP1 Gene ID (NCBI): 79753 RRID: AB_3670708 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q8TAD8 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.