Proteintech, 82972-1-RR, Cytokeratin 1-specific Recombinant monoclonal antibody

Size: 20ul / 100ul The Cytokeratin 1-specific (82972-1-RR) by Proteintech is a Recombinant antibody targeting Cytokeratin 1-specific in WB, FC (Intra), ELISA applications with reactivity to human, mouse, rat samples 82972-1-RR targets Cytokeratin 1-specific in WB, FC (Intra), ELISA applications and shows reactivity with human, mouse, rat samples. Tested Applications Positive WB detected in: mouse skin tissue, rat skin tissue Positive FC (Intra) detected in: A431 cells Recommended dilution Western Blot (WB): WB : 1:5000-1:50000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Background Information Keratins are a large family of proteins that form the intermediate filament cytoskeleton of epithelial cells, which are classified into two major sequence types. Type I keratins are a group of acidic intermediate filament proteins, including K9-K23, and the hair keratins Ha1-Ha8. Type II keratins are the basic or neutral courterparts to the acidic type I keratins, including K1-K8, and the hair keratins, Hb1-Hb6. Keratin-1 is type II keratin. It is a specific marker for terminal differentiation in mammalian epidermis. It is specifically expressed in the spinous and granular layers of the epidermis with family member KRT10 and mutations in these genes have been associated with bullous congenital ichthyosiform erythroderma. Specification Tested Reactivity: human, mouse, rat Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: keratin 1 Calculated Molecular Weight: 66 kDa Observed Molecular Weight: 67 kDa GenBank Accession Number: NM_006121 Gene Symbol: KRT1 Gene ID (NCBI): 3848 RRID: AB_3670720 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P04264 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.