Proteintech, 82996-4-RR, RILP Recombinant monoclonal antibody

Size: 20ul / 100ul The RILP (82996-4-RR) by Proteintech is a Recombinant antibody targeting RILP in WB, IHC, FC (Intra), ELISA applications with reactivity to human samples 82996-4-RR targets RILP in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: HeLa cells, HepG2 cells, MCF-7 cells Positive IHC detected in: human ovarian cancerNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive FC (Intra) detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:1000-1:4000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Affinity: K D =1.04 x 10 -11 M K Off =2.33 x 10 -5 M K On =2.05 x 10 6 M Immunogen: CatNo: Ag4472 Product name: Recombinant human RILP protein Source: e coli. -derived, T-HIS Tag: 6*His Domain: 102-401 aa of BC031621 Sequence: LRAGPQEERALLRQLKEVTDRQRDELRAHNRDLRQRGQETEALQEQLQRLLLVNAELRHKLAAMQTQLRAAQDRERERQQPGEAATPQAKERARGQAGRPGHQHGQEPEWATAGAGAPGNPEDPAEAAQQLGRPSEAGQCRFSREEFEQILQERNELKAKVFLLKEELAYFQRELLTDHRVPGLLLEAMKVAVRKQRKKIKAKMLGTPEEAESSEDEAGPWILLSDDKGDHPPPPESKIQSFFGLWYRGKAESSEDETSSPAPSKLGGEEEAQPQSPAPDPPCSALHEHLCLGASAAPEA Predict reactive species Full Name: Rab interacting lysosomal protein Calculated Molecular Weight: 401 aa, 41 kDa Observed Molecular Weight: 50 kDa GenBank Accession Number: BC031621 Gene Symbol: RILP Gene ID (NCBI): 83547 RRID: AB_3670743 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96NA2 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.