Proteintech, 83017-2-RR, CTAGE5 Recombinant monoclonal antibody

Size: 20ul / 100ul The CTAGE5 (83017-2-RR) by Proteintech is a Recombinant antibody targeting CTAGE5 in WB, IF/ICC, FC (Intra), ELISA applications with reactivity to human samples 83017-2-RR targets CTAGE5 in WB, IF/ICC, FC (Intra), ELISA applications and shows reactivity with human samples. Tested Applications Positive WB detected in: U-87 MG cells, HEK-293T cells, PC-3 cells, HeLa cells, Jurkat cells Positive IF/ICC detected in: HepG2 cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Western Blot (WB): WB : 1:1000-1:8000 Immunofluorescence (IF)/ICC: IF/ICC : 1:125-1:500 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension Background Information Cutaneous T cell lymphoma-associated antigen 5 (CTAGE5) is a member of the cTAGE family. CTAGE5 is a tumor-associated antigen and is overexpressed in various cancer cell lines. CTAGE5 consists of a signal anchor motif, a single transmembrane domain, two coiled-coil motifs, and a proline-rich domain (PRD) from the N-terminus. CTAGE5 interacts with TANGO1 through its second coil region to form a cargo receptor complex (PMID: 21525241). Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: CTAGE family, member 5 Calculated Molecular Weight: 92 kDa Observed Molecular Weight: 110 kDa GenBank Accession Number: NM_005930 Gene Symbol: CTAGE5 Gene ID (NCBI): 4253 RRID: AB_3670760 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q96PC5 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.