Proteintech, 83041-1-RR, Acetyl-Histone H2A (Lys9) Recombinant monoclonal antibody

Size: 20ul / 100ul The Acetyl-Histone H2A (Lys9) (83041-1-RR) by Proteintech is a Recombinant antibody targeting Acetyl-Histone H2A (Lys9) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications with reactivity to human, mouse samples 83041-1-RR targets Acetyl-Histone H2A (Lys9) in WB, IHC, IF/ICC, Dot Blot, ELISA, ChIP-qPCR applications and shows reactivity with human, mouse samples. Tested Applications Positive WB detected in: Trichostatin A treated NIH/3T3 cells Positive IHC detected in: mouse lung tissue, human placenta tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive Dot Blot detected in: peptide Positive ChIP-qPCR detected in: HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 Immunohistochemistry (IHC): IHC : 1:250-1:1000 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 DOT BLOT: DOT BLOT : 1:10-1:100 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information Histone H2A is a core component of nucleosome. Histone variants contribute to chromatin complexity by creating specialized nucleosomes. Within nucleosomes, either one canonical H2A or both of them can be exchanged with a particular variant (heterotypic and homotypic nucleosomes, respectively), and such changes can have profound influences on nucleosome stability and biological outcome. Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H2ae Calculated Molecular Weight: 14 kDa Observed Molecular Weight: 14 kDa GenBank Accession Number: BC093836 Gene Symbol: HIST1H2AE Gene ID (NCBI): 3012 RRID: AB_3670771 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P04908 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.