Proteintech, 83041-8-RR, Phospho-Histone H2A (Thr120) Recombinant monoclonal antibody

Size: 20ul / 100ul The Phospho-Histone H2A (Thr120) (83041-8-RR) by Proteintech is a Recombinant antibody targeting Phospho-Histone H2A (Thr120) in WB, ELISA, ChIP-qPCR applications with reactivity to human samples 83041-8-RR targets Phospho-Histone H2A (Thr120) in WB, ELISA, ChIP-qPCR applications and shows reactivity with human samples. Tested Applications Positive WB detected in: Nocodazole and Calyculin A treated HeLa cells Positive ChIP-qPCR detected in: Nocodazole (100 ng/ml) for 16 h HeLa cells Recommended dilution Western Blot (WB): WB : 1:2000-1:10000 CHIP-QPCR: CHIP-QPCR : 1:10-1:100 Background Information Phospho-Histone H2A (Thr120) refers to the phosphorylation of threonine 120 on histone H2A, a modification that is increasingly recognized for its roles in chromatin organization, gene regulation, and disease development, particularly in oncogenesis and bone remodeling. The phosphorylation is predominantly associated with gene silencing. High levels of H2AT120p correlate with increased cell proliferation, tumor growth, and poor prognosis, underscoring its role as an oncogenic signal. Specification Tested Reactivity: human Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: Peptide Predict reactive species Full Name: histone cluster 1, H2ae Calculated Molecular Weight: 14 kDa Observed Molecular Weight: 15 kDa GenBank Accession Number: BC093836 Gene Symbol: HIST1H2AE Gene ID (NCBI): 3012 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: P04908 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.