Proteintech, 83052-7-RR, SURF1 Recombinant monoclonal antibody

Size: 20ul / 100ul The SURF1 (83052-7-RR) by Proteintech is a Recombinant antibody targeting SURF1 in IHC, IF/ICC, FC (Intra), IP, ELISA applications with reactivity to human, mouse samples 83052-7-RR targets SURF1 in IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse samples. Tested Applications Positive IP detected in: HeLa cells Positive IHC detected in: mouse heart tissue, human liver cancer tissueNote: suggested antigen retrieval withTE buffer pH 9.0;(*) Alternatively, antigen retrieval may be performed withcitrate buffer pH 6.0 Positive IF/ICC detected in: HeLa cells Positive FC (Intra) detected in: HepG2 cells Recommended dilution Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate Immunohistochemistry (IHC): IHC : 1:200-1:800 Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800 Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension Specification Tested Reactivity: human, mouse Host / Isotype: Rabbit / IgG Class: Recombinant Type: Antibody Immunogen: CatNo: Ag3959 Product name: Recombinant human SURF1 protein Source: e coli. -derived, PGEX-4T Tag: GST Domain: 1-300 aa of BC028314 Sequence: MAAVAALQLGLRAAGLGRAPASAAWRSVLRVSPRPGVAWRPSRCGSSAAEASATKAEDDSFLQWVLLLIPVTAFGLGTWQVQRRKWKLNLIAELESRVLAEPVPLPADPMELKNLEYRPVKVRGCFDHSKELYMMPRTMVDPVREAREGGLISSSTQSGAYVVTPFHCTDLGVTILVNRGFVPRKKVNPETRQKGQIEGEVDLIGMVRLTETRQPFVPENNPERNHWHYRDLEAMARITGAEPIFIDANFQSTVPGGPIGGQTRVTLRNEHLQYIVTWYGLSAATSYLWFKKFLRGTPGV Predict reactive species Full Name: surfeit 1 Calculated Molecular Weight: 33 kDa Observed Molecular Weight: 30-31 kDa GenBank Accession Number: BC028314 Gene Symbol: SURF1 Gene ID (NCBI): 6834 RRID: AB_3670777 Conjugate: Unconjugated Form: Liquid Purification Method: Protein A purification UNIPROT ID: Q15526 Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20 o C storage. 20ul sizes contain 0.1% BSA.